Anticorps Recombinant de lapin anti-MTAP

• Phare
• Validé par KD/KO

MTAP Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240030A3

N° de cat : 83005-1-RR

Synonymes

240030A3, 5'-methylthioadenosine phosphorylase, c86fus, EC:2.4.2.28, MSAP

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Galerie de données de validation

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  WB analysis of HEK-293 using 83005-1-RR

  WB result of MTAP antibody (83005-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTAP transfected HEK-293 cells.

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  WB analysis using 83005-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83005-1-RR (MTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83005-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83005-1-RR against Human MTAP were performed. The affinity constant is 0.782 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HCT 116, cellules HEK-293, cellules HT-29, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83005-1-RR cible MTAP dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MTAP Protéine recombinante Ag2051
• Nom complet: methylthioadenosine phosphorylase
• Masse moléculaire calculée: 283 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC018625
• Symbole du gène: MTAP
• Identification du gène (NCBI): 4507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

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