Anticorps Polyclonal de lapin anti-MTCL2

MTCL2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 33016-1-AP

Synonymes

80-kDa SOGA fragment, C20orf117, C-terminal 80 kDa form, KIAA0889, Microtubule cross-linking factor 2

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Galerie de données de validation

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  WB analysis using 33016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33016-1-AP (C20orf117 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 33016-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 33016-1-AP (C20orf117 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cerveau humain fœtal

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

33016-1-AP cible MTCL2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTCL2 Protéine recombinante Ag35888
• Nom complet: chromosome 20 open reading frame 117
• Masse moléculaire calculée: 159kDa
• Poids moléculaire observé: 183 kDa, 50 kDa
• Numéro d’acquisition GenBank: NM_080627
• Symbole du gène: C20orf117
• Identification du gène (NCBI): 140710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The coiled-coil protein MTCL2 preferentially acts on the perinuclear microtubules accumulated around the Golgi. MTCL2 associates with the Golgi membrane through the N-terminal coiled-coil region and directly binds microtubules through the conserved C-terminal domain without promoting microtubule stabilization. Knockdown of MTCL2 significantly impaired microtubule accumulation around the Golgi, as well as the compactness of the Golgi ribbon assembly structure. MTCL2 forms parallel oligomers through homo-interaction of the central coiled-coil motifs, and promotes asymmetric microtubule organization by crosslinking microtubules on the Golgi membrane. Results of in vitro wound healing assays further suggest that this function of MTCL2 enables integration of the centrosomal and Golgi-associated microtubules on the Golgi membrane, supporting directional migration. Additionally, the results demonstrated the involvement of CLASPs and giantin in mediating the Golgi association of MTCL2 (PMID: 35543016).

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