Anticorps Monoclonal anti-MTH1

MTH1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D7G4

N° de cat : 67443-1-PBS

Synonymes

NUDT1, 2D7G4, 2-hydroxy-dATP diphosphatase, 7,8-dihydro-8-oxoguanine triphosphatase, 8 oxo dGTPase

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Galerie de données de validation

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  WB analysis using 67443-1-Ig (same clone as 67443-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67443-1-Ig (MTH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 67443-1-Ig (same clone as 67443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67443-1-Ig (same clone as 67443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67443-1-Ig (same clone as 67443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67443-1-Ig (same clone as 67443-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67443-1-Ig (MTH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67443-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50783-2

  Cytometric bead array standard curve of MP50783-2, NUDT1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67443-1-PBS. Detection antibody: 67443-4-PBS. Standard:Ag9802. Range: 0.195-100 ng/mL.

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Informations sur le produit

67443-1-PBS cible MTH1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTH1 Protéine recombinante Ag9802
• Nom complet: nudix (nucleoside diphosphate linked moiety X)-type motif 1
• Masse moléculaire calculée: 179aa,20 kDa; 197aa,23 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC014618
• Symbole du gène: MTH1
• Identification du gène (NCBI): 4521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.

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