Anticorps Polyclonal de lapin anti-MTHFD1

• Phare
• Validé par KD/KO

MTHFD1 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 10794-1-AP

Synonymes

MTHFC, MTHFD, MTHFD1

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Galerie de données de validation

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  WB analysis using 10794-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10794-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10794-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 10794-1-AP (MTHFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10794-1-AP

  IP Result of anti-MTHFD1 (IP:10794-1-AP, 5ug; Detection:10794-1-AP 1:600) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10794-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10794-1-AP (MTHFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HuH-7, cellules K-562, tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below

Informations sur le produit

10794-1-AP cible MTHFD1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTHFD1 Protéine recombinante Ag1225
• Nom complet: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• Masse moléculaire calculée: 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC009806
• Symbole du gène: MTHFD1
• Identification du gène (NCBI): 4522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Publications

Species	Application	Title
human	WB	Nature; Oxidative stress inhibits distant metastasis by human melanoma cells.; Authors - Elena Piskounova ; KD Validated
human	WB	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
human	WB	Redox Biol; Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.; Authors - Chang Shao
human	WB	Proc Natl Acad Sci U S A; Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.; Authors - Danielle E Anderson ; KD Validated
mouse	WB	Dev Cell; Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.; Authors - Yejun Zou
human	WB	Mol Oncol; Cysteine is a limiting factor for glioma proliferation and survival.; Authors - Victor Ruiz-Rodado

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: liver, kidney, Hippocampus

MTHFD1 Antibody Western Blot, validation (1:500 dilution) in liver, kidney, Hippocampus (Cat no:10794-1-AP)