Anticorps Monoclonal anti-MTHFD1

• Phare
• Validé par KD/KO

MTHFD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B1G3

N° de cat : 67670-1-PBS

Synonymes

MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic

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Galerie de données de validation

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  WB analysis using 67670-1-Ig (same clone as 67670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67670-1-Ig (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67670-1-Ig (same clone as 67670-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67670-1-PBS cible MTHFD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTHFD1 Protéine recombinante Ag24553
• Nom complet: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• Masse moléculaire calculée: 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC009806
• Symbole du gène: MTHFD1
• Identification du gène (NCBI): 4522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

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