Anticorps Monoclonal anti-MTHFD1L

MTHFD1L Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G1F2

N° de cat : 68321-1-PBS

Synonymes

MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3, 1G1F2

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Galerie de données de validation

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  WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  WB analysis using 68321-1-Ig (same clone as 68321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68321-1-Ig (MTHFD1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68321-1-Ig (same clone as 68321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68321-1-Ig (MTHFD1L antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 68321-1-Ig (same clone as 68321-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MTHFD1L Monoclonal antibody (68321-1-Ig, Clone:1G1F2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68321-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50271-2

  Cytometric bead array standard curve of MP50271-2, MTHFD1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68321-1-PBS. Detection antibody: 68321-3-PBS. Standard:Ag9061. Range: 0.195-50 ng/mL.

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Informations sur le produit

68321-1-PBS cible MTHFD1L dans les applications de WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTHFD1L Protéine recombinante Ag9061
• Nom complet: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• Masse moléculaire calculée: 978 aa, 106 kDa
• Poids moléculaire observé: 106 kDa
• Numéro d’acquisition GenBank: BC017477
• Symbole du gène: MTHFD1L
• Identification du gène (NCBI): 25902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.

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