Anticorps Monoclonal anti-MTHFD2

MTHFD2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B4B6

N° de cat : 68524-1-PBS

Synonymes

MTHFD2

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Galerie de données de validation

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  WB analysis using 68524-1-Ig (same clone as 68524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68524-1-Ig (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68524-1-Ig (same clone as 68524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68524-1-Ig (MTHFD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68524-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68524-1-PBS cible MTHFD2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTHFD2 Protéine recombinante Ag33605
• Nom complet: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Masse moléculaire calculée: 350 aa, 38 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: BC017054
• Symbole du gène: MTHFD2
• Identification du gène (NCBI): 10797
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

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