Anticorps Recombinant de lapin anti-MTHFD2

MTHFD2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251431F2

N° de cat : 86608-3-RR

Synonymes

Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, Methenyltetrahydrofolate cyclohydrolase

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Galerie de données de validation

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  WB analysis using 86608-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86608-3-RR (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 86608-3-RR

  rat testis tissue were subjected to SDS PAGE followed by western blot with 86608-3-RR (MTHFD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86608-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTHFD2 antibody (86608-3-RR, Clone: 251431F2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86608-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86608-3-RR against Human MTHFD2 were performed. The affinity constant is 2.95 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules Jurkat, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86608-3-RR cible MTHFD2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MTHFD2 Protéine recombinante Ag2911
• Nom complet: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Masse moléculaire calculée: 350 aa, 38 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: BC017054
• Symbole du gène: MTHFD2
• Identification du gène (NCBI): 10797
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

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