Anticorps Monoclonal anti-MTHFR

MTHFR Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F7A3

N° de cat : 66612-1-PBS

Synonymes

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 1F7A3

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Galerie de données de validation

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  WB analysis of LNCaP using 66612-1-Ig (same clone as 66612-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66612-1-Ig (same clone as 66612-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66612-1-Ig (same clone as 66612-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66612-1-Ig (same clone as 66612-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66612-1-Ig (MTHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66612-1-Ig (same clone as 66612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66612-1-Ig (same clone as 66612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66612-1-Ig (MTHFR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66612-1-Ig (same clone as 66612-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTHFR antibody (66612-1-Ig, Clone: 1F7A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66612-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66612-1-PBS cible MTHFR dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTHFR Protéine recombinante Ag14970
• Nom complet: 5,10-methylenetetrahydrofolate reductase (NADPH)
• Masse moléculaire calculée: 656 aa, 75 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC053509
• Symbole du gène: MTHFR
• Identification du gène (NCBI): 4524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

5,10-Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in the folate metabolic pathway with a key role in generating methyl groups. MTHFR reduces 5,10-methylenetetrahydrofolate (5,10-methyleneTHF) to 5-methylTHF, the primary carbon donor for methionine production from homocysteine. In humans and mice studies, mutations and polymorphisms in MTHFR are associated with impaired spermatogenesis and male infertility. It has been reported that MTHFR is highly phosphorylated under unperturbed conditions and T34 is the priming phosphorylation site. MTHFR gene has two promoters and isoform (70 kDa and 77 kDa). Although the major form of MTHFR is 77kDa in most tissues, a second isoform of 70kDa was observed after western blotting of human fetal liver and porcine liver. (PMID: 24769206, PMID: 34128976, PMID: 18615588)

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