Anticorps Recombinant de lapin anti-MTHFR

MTHFR Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240348H7

N° de cat : 83372-4-PBS

Synonymes

EC:1.5.1.53, Methylenetetrahydrofolate reductase (NADPH)

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00372-1

  Sandwich ELISA standard curve of MP00372-1, Human MTHFR Recombinant Matched Antibody Pair - PBS only. 83372-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14970. 83372-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP00372-1

  K-562, Hela and HepG2 cell lysates were measured. The human MTHFR concentration of detected samples was determined to be 88.86 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate, 57.89 ng/mL (based on a 3.2 mg/mL extract load) in Hela cell lysate and 55.55 ng/mL (based on a 5 mg/mL extract load) in HepG2 cell lysate.

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  Cytometric bead array standard curve of MP00372-1

  Cytometric bead array standard curve of MP00372-1, MTHFR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83372-5-PBS. Detection antibody: 83372-4-PBS. Standard: Ag14970. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83372-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83372-4-PBS against Human MTHFR was performed. The affinity constant is 0.759 nM.

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Informations sur le produit

83372-4-PBS cible MTHFR dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MTHFR Protéine recombinante Ag14970
• Nom complet: 5,10-methylenetetrahydrofolate reductase (NADPH)
• Masse moléculaire calculée: 656 aa, 75 kDa
• Numéro d’acquisition GenBank: BC053509
• Symbole du gène: MTHFR
• Identification du gène (NCBI): 4524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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