Anticorps Polyclonal de lapin anti-MTMR4

MTMR4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13488-1-PBS

Synonymes

EC:3.1.3.48, FYVE domain-containing dual specificity protein phosphatase 2, FYVE DSP2, FYVE-DSP2, KIAA0647

---

Galerie de données de validation

• 

  WB analysis using 13488-1-AP (same clone as 13488-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13488-1-AP (MTMR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Hepatocellular carcinoma using 13488-1-AP (same clone as 13488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Hepatocellular carcinoma using 13488-1-AP (same clone as 13488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded hepatocellular carcinoma tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 13488-1-AP (same clone as 13488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13488-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 13488-1-AP (same clone as 13488-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13488-1-AP (MTMR4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13488-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13488-1-AP (same clone as 13488-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (13488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13488-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

13488-1-PBS cible MTMR4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTMR4 Protéine recombinante Ag4310
• Nom complet: myotubularin related protein 4
• Masse moléculaire calculée: 1195 aa, 133 kDa
• Poids moléculaire observé: 133 kDa
• Numéro d’acquisition GenBank: BC035609
• Symbole du gène: MTMR4
• Identification du gène (NCBI): 9110
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).

{{ptg:RelatedPrimaryAntibodies}}