Anticorps Recombinant de lapin anti-MTMR4

• Phare
• Validé par KD/KO

MTMR4 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241304E11

N° de cat : 84328-1-RR

Synonymes

KIAA0647, FYVE-DSP2, FYVE DSP2, FYVE domain-containing dual specificity protein phosphatase 2, EC:3.1.3.48

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 84328-1-RR

  WB result of MTMR4 antibody (84328-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR4 transfected Jurkat cells.

• 

  WB analysis using 84328-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of Human Hepatocellular Cancer using 84328-1-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular Cancer slide using 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human Hepatocellular Cancer using 84328-1-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular Cancer slide using 84328-1-RR (MTMR4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 84328-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (84328-1-RR, Clone: 241304E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 84328-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84328-1-RR against Human MTMR4 were performed. The affinity constant is 2.06 nM.

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84328-1-RR cible MTMR4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MTMR4 Protéine recombinante Ag4310
• Nom complet: myotubularin related protein 4
• Masse moléculaire calculée: 1195 aa, 133 kDa
• Poids moléculaire observé: 160-170 kDa
• Numéro d’acquisition GenBank: BC035609
• Symbole du gène: MTMR4
• Identification du gène (NCBI): 9110
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048). MTMR4 is an intracellular protein with a calculated molecular mass of 133 kDa, and detected molecular weight in the papers is about 160-170 kDa, which may be due to post-translational modification (PMID: 19125695, PMID: 20736309).

{{ptg:RelatedPrimaryAntibodies}}