Anticorps Recombinant de lapin anti-MTMR9

MTMR9 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240167G7

N° de cat : 83694-4-PBS

Synonymes

240167G7, C8orf9, Inactive phosphatidylinositol 3-phosphatase 9, LIP STYX, MTMR8

---

Galerie de données de validation

• 

  WB analysis using 83694-4-RR (same clone as 83694-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83694-4-RR (MTMR9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83694-4-RR (same clone as 83694-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR9 antibody (83694-4-RR, Clone: 240167G7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 83694-4-RR (same clone as 83694-4-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83694-4-RR (same clone as 83694-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83694-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83694-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83694-4-RR against Human MTMR9 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

83694-4-PBS cible MTMR9 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MTMR9 Protéine recombinante Ag34134
• Nom complet: myotubularin related protein 9
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: ~60 kDa
• Numéro d’acquisition GenBank: BC022003
• Symbole du gène: MTMR9
• Identification du gène (NCBI): 66036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598).

{{ptg:RelatedPrimaryAntibodies}}