Anticorps Recombinant de lapin anti-MTMR9

MTMR9 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240167G7

N° de cat : 83694-4-RR

Synonymes

240167G7, C8orf9, Inactive phosphatidylinositol 3-phosphatase 9, LIP STYX, MTMR8

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Galerie de données de validation

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  WB analysis using 83694-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83694-4-RR (MTMR9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83694-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR9 antibody (83694-4-RR, Clone: 240167G7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 83694-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 83694-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MTMR9 Recombinant antibody (83694-4-RR, Clone:240167G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83694-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83694-4-RR against Human MTMR9 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HEK-293, cellules HeLa, cellules HepG2, cellules U2OS
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83694-4-RR cible MTMR9 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MTMR9 Protéine recombinante Ag34134
• Nom complet: myotubularin related protein 9
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: ~60 kDa
• Numéro d’acquisition GenBank: BC022003
• Symbole du gène: MTMR9
• Identification du gène (NCBI): 66036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598).

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