Anticorps Polyclonal de lapin anti-mTOR

• Phare
• Validé par KD/KO

mTOR Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

189

N° de cat : 20657-1-AP

Synonymes

FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

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Galerie de données de validation

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  WB analysis of MCF-7 using 20657-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 20657-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20657-1-AP (MTOR Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 20657-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20657-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20657-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20657-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20657-1-AP (mTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20657-1-AP

  Immunofluorescent analysis of HeLa cells using 20657-1-AP (MTOR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules MDA-MB-453s
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 179 publications below
• IHC: See 8 publications below
• IF: See 8 publications below

Informations sur le produit

20657-1-AP cible mTOR dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, poulet, souris, Hamster, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: FK506 binding protein 12-rapamycin associated protein 1
• Masse moléculaire calculée: 289 kDa
• Numéro d’acquisition GenBank: NM_004958
• Symbole du gène: FRAP1
• Identification du gène (NCBI): 2475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359). The antibody is specific to MTOR.

Publications

Species	Application	Title
human	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
human	WB	Cell Death Differ; PIWIL2 interacting with IKK to regulate autophagy and apoptosis in esophageal squamous cell carcinoma.; Authors - Xu Zhao
human	WB	Autophagy; FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma.; Authors - Tianyu Han
rat	IF	Autophagy; Autophagy and post-ischemic conditioning in retinal ischemia.; Authors - Biji Mathew
human	WB	Int J Biol Macromol; Novel soybean polypeptide dglycin alleviates atherosclerosis in apolipoprotein E-deficient mice; Authors - Han Zhao

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