Anticorps Polyclonal de lapin anti-MUC16/CA125

• Phare
• Validé par KD/KO

MUC16/CA125 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20077-1-PBS

Synonymes

MUC16, MUC16,CA125, MUC-16, MUC 16, CA125

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Galerie de données de validation

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  WB analysis of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 20077-1-AP (same clone as 20077-1-PBS)

  IP result of anti-MUC16,CA125 (IP:20077-1-AP, 4ug; Detection:20077-1-AP 1:600) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20077-1-PBS cible MUC16/CA125 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mucin 16, cell surface associated
• Masse moléculaire calculée: 1519 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: NM_024690
• Symbole du gène: MUC16
• Identification du gène (NCBI): 94025
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators. The antibody recognizes the C-term of MUC16.

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