Anticorps Polyclonal de lapin anti-MUC2

MUC2 Polyclonal Antibody for IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27675-1-PBS

Synonymes

MUC 2, MUC-2, Mucin 2, SMUC

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Galerie de données de validation

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  IHC staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 27675-1-AP (same clone as 27675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 27675-1-AP (same clone as 27675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IHC staining of rat small intestine using 27675-1-AP (same clone as 27675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 27675-1-AP (same clone as 27675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 27675-1-AP (same clone as 27675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27675-1-AP (MUC2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IF Staining of rat small intestine using 27675-1-AP (same clone as 27675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IF Staining of rat small intestine using 27675-1-AP (same clone as 27675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat small intestine using 27675-1-AP (same clone as 27675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004), smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon using 27675-1-AP (same clone as 27675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse colon using 27675-1-AP (same clone as 27675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IF Staining of SW480 using 27675-1-AP (same clone as 27675-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed SW480 cells using MUC2 antibody (27675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 27675-1-AP (same clone as 27675-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MUC2 antibody (27675-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 27675-1-AP (same clone as 27675-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.13 ug MUC2 Polyclonal antibody (27675-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27675-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27675-1-PBS cible MUC2 dans les applications de IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MUC2 Protéine recombinante Ag25800
• Nom complet: mucin 2, oligomeric mucus/gel-forming
• Masse moléculaire calculée: 540 kDa
• Numéro d’acquisition GenBank: M94132
• Symbole du gène: MUC2
• Identification du gène (NCBI): 4583
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

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