Anticorps Monoclonal anti-MUC20

MUC20 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

canin, Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G9B2

N° de cat : 68714-1-Ig

Synonymes

UNQ2782/PRO7170, Mucin-20, MUC-20, MUC 20, KIAA1359

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Galerie de données de validation

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  WB analysis using 68714-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68714-1-Ig (MUC20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68714-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MUC20 antibody (68714-1-Ig, Clone: 1G9B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules MDCK, cellules A549, cellules HEK-293
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68714-1-Ig cible MUC20 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MUC20 Protéine recombinante Ag31840
• Nom complet: mucin 20, cell surface associated
• Masse moléculaire calculée: 55 kDa, 71 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC029267
• Symbole du gène: MUC20
• Identification du gène (NCBI): 200958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MUC20 contains serine, threonine, and proline-rich repeats in its extracellular domain and is likely to be extensively O-glycosylated. MUC20 blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway and inhibiting HGF-induced proliferation of MMP1 and MMP9 expression (PMID: 15314156). MUC20 is highly expressed in the kidney and localized in the proximal tubules but not in the glomerulus or distal tubules(PMID: 14565953). MUC20 was detected in most of the male urogenital tract epithelia, except for epididymis(PMID: 16997931). The expression of MUC20 is up-regulated in the kidneys of patients with immunoglobulin A nephropathy, in an animal model of lupus nephritis, and in mice with acute renal injury caused by cisplatin administration or unilateral ureteral obstruction(PMID: 14565953).

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