Anticorps Polyclonal de lapin anti-MUC8

MUC8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 55489-1-AP

Synonymes

mucin 8

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Galerie de données de validation

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  WB analysis of A549 using 55489-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55489-1-AP (MUC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55489-1-AP (MUC8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 55489-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 55489-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MUC8 antibody (55489-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

55489-1-AP cible MUC8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mucin 8
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: U14383
• Symbole du gène: MUC8
• Identification du gène (NCBI): 4590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mucin glycoproteins (Mucins) are major constituents of the glycocalyx that covers mucosal epithelium. MUC8 is one of the major mucins in the ethmoid mucosa and is upregulated by chronic inflammation. TNFa, IL-1b and a combination of both can significantly increase Mucin 8 mRNA levels, suggesting that a mixture of inflammatory mediators can synergistically increase secretion of mucin in human nasal epithelium. This antibody is specific to MUC8.

Publications

Species	Application	Title
human	IHC,WB	Diagnostics (Basel); The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?; Authors - Martin Schicht

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