Anticorps Monoclonal anti-MUM1

MUM1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3B1D2

N° de cat : 60353-1-Ig

Synonymes

EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere

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Galerie de données de validation

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  WB analysis of HEK-293 using 60353-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 60353-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 60353-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60353-1-Ig (MUM1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 60353-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MUM1 antibody (60353-1-Ig, Clone: 3B1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of A375 using 60353-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 60353-1-Ig (MUM1 antibody), at dilution of 1:50 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A375, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa, cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

60353-1-Ig cible MUM1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MUM1 Protéine recombinante Ag3371
• Nom complet: melanoma associated antigen (mutated) 1
• Masse moléculaire calculée: 18 kDa, 79 kDa
• Poids moléculaire observé: 78-80 kDa
• Numéro d’acquisition GenBank: BC008098
• Symbole du gène: MUM1
• Identification du gène (NCBI): 84939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.

Publications

Species	Application	Title
human	IHC	Front Oncol; Clinical Impact of the Histopathological Index and Neuroimaging Features Status in Primary Central Nervous System Diffuse Large B-Cell Lymphoma: A Single-Center Retrospective Analysis of 51 Cases.; Authors - Zhou Qi

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