Anticorps Polyclonal de lapin anti-MUS81

• Phare
• Validé par KD/KO

MUS81 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 11018-1-AP

Synonymes

EC:3.1.22.-

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Galerie de données de validation

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  WB analysis using 11018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11018-1-AP (MUS81 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11018-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11018-1-AP (MUS81 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 11018-1-AP

  Immunofluorescent analysis of MCF-7 cells, using MUS81 antibody 11018-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

11018-1-AP cible MUS81 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MUS81 Protéine recombinante Ag1476
• Nom complet: MUS81 endonuclease homolog (S. cerevisiae)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 52-72 kDa
• Numéro d’acquisition GenBank: BC009999
• Symbole du gène: MUS81
• Identification du gène (NCBI): 80198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).

Publications

Species	Application	Title
human	WB,IHC	Cell; EMSY inhibits homologous recombination repair and the interferon response, promoting lung cancer immune evasion.; Authors - Antonio Marzio ; KD Validated
	WB	Elife; 53BP1 and BRCA1 control pathway choice for stalled replication restart.; Authors - Yixi Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Arnaud (Verified Customer) (09-26-2025)

2 bands at 70kDa, work well at 1:1000 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for WB
• Cell Tissue Type: HeLa

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