Anticorps Polyclonal de lapin anti-Methylmalonyl Coenzyme A mutase/MUT

Methylmalonyl Coenzyme A mutase/MUT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17034-1-PBS

Synonymes

MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl Coenzyme A mutase

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Galerie de données de validation

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  WB analysis using 17034-1-AP (same clone as 17034-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 17034-1-AP (same clone as 17034-1-PBS)

  IP result of anti-Methylmalonyl Coenzyme A mutase/MUT (IP:17034-1-AP, 4ug; Detection:17034-1-AP 1:500) with HepG2 cells lysate 1800ug. This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17034-1-PBS cible Methylmalonyl Coenzyme A mutase/MUT dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Methylmalonyl Coenzyme A mutase/MUT Protéine recombinante Ag10523
• Nom complet: methylmalonyl Coenzyme A mutase
• Masse moléculaire calculée: 750 aa, 83 kDa
• Poids moléculaire observé: 78 kDa
• Numéro d’acquisition GenBank: BC016282
• Symbole du gène: MUT
• Identification du gène (NCBI): 4594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids (PMID: 15647267). Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA (PMID: 30428564), resulting in life-threatening metabolic acidosis, respiratory distress, neurological impairment, hyperammonemia, and ketosis (PMID: 32679819). MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine (PMID: 23041189).

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