Anticorps Polyclonal de lapin anti-MUTYH

• Phare
• Validé par KD/KO

MUTYH Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 19650-1-AP

Synonymes

hMYH, MutY homolog, mutY homolog (E. coli), MUTYH, MYH

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Galerie de données de validation

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  WB analysis of HeLa using 19650-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19650-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 19650-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19650-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19650-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19650-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19650-1-AP (MUTYH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19650-1-AP

  IP Result of anti-MUTYH (IP:19650-1-AP, 4ug; Detection:19650-1-AP 1:500) with HeLa cells lysate 3440ug.

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  IHC staining of human kidney using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

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  IHC staining of mouse liver using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19650-1-AP (MUTYH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 19650-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19650-1-AP (MUTYH Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules Jurkat, cellules K-562, cellules Raji, tissu de thymus de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

19650-1-AP cible MUTYH dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MUTYH Protéine recombinante Ag7526
• Nom complet: mutY homolog (E. coli)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 53-64 kDa
• Numéro d’acquisition GenBank: BC003178
• Symbole du gène: MUTYH
• Identification du gène (NCBI): 4595
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MUTYH(MutY homolog) is also named as MYH and belongs to the Nth/MutY family. The gene enconde an A/G-specific adenine DNA glycosylase that it is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities(PMID:10684930). Defects in MUTYH are a cause of familial adenomatous polyposis type 2 (FAP2) and gastric cancer (GASC). It has 6 isoforms produced by alternative splicing with the molecular weight between 57 kDa and 60 kDa.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; MUTYH Actively Contributes to Microglial Activation and Impaired Neurogenesis in the Pathogenesis of Alzheimer's Disease; Authors - Yuri Mizuno
human	WB	Oncol Rep; Downregulation of MUTYH contributes to cisplatin‑resistance of esophageal squamous cell carcinoma cells by promoting Twist‑mediated EMT.; Authors - Yanxia Guo ; KD Validated
human	WB	Cell Death Dis; Potent and specific MTH1 inhibitors targeting gastric cancer.; Authors - Wenjuan Zhou