Anticorps Polyclonal de lapin anti-MVK

MVK Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 12228-1-AP

Synonymes

MVLK, MK, Mevalonate kinase, LRBP, EC:2.7.1.36

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Galerie de données de validation

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  WB analysis using 12228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse uterus using 12228-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 12228-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12228-1-AP (MVK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 12228-1-AP

  Immunofluorescent analysis of PC-3 cells using 12228-1-AP (MVK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HepG2, cellules Jurkat, cellules PC-3, tissu utérin de souris
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below

Informations sur le produit

12228-1-AP cible MVK dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MVK Protéine recombinante Ag2865
• Nom complet: mevalonate kinase
• Masse moléculaire calculée: 396 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC016140
• Symbole du gène: MVK
• Identification du gène (NCBI): 4598
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MVK (Mevalonate kinase) is a 42 kDa cytoplasmic protein that belongs to the GHMP kinase family. Mevalonate kinase catalyzes the ATP-dependent phosphorylation of mevalonic acid to form mevalonate 5-phosphate. Defects in mevalonate kinase can cause mevalonic aciduria (MEVA). It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy and ataxia.

Publications

Species	Application	Title
mouse	WB	Bioorg Chem; Gypenoside L inhibits hepatocellular carcinoma by targeting the SREBP2-HMGCS1 axis and enhancing immune response; Authors - Man-Yu Xiao
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing
mouse	WB	Curr Issues Mol Biol; Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.; Authors - Audrey Basque
sheep	WB	Anim Reprod Sci; Acute heat stress regulates estradiol synthesis in ovine ovarian granulosa cells through the SREBPs/MVK-LHR pathway; Authors - Yinxia Li
human	WB	Exp Ther Med; Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.; Authors - Min Li
mouse	WB	Toxicol In Vitro; Sinapic and ferulic acid phenethyl esters increase the expression of steroidogenic genes in MA-10 tumor Leydig cells; Authors - Audrey Basque

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