Anticorps Polyclonal de lapin anti-MYB/c-Myb

MYB/c-Myb Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 17800-1-AP

Synonymes

c myb_CDS, Cmyb, c-Myb, efg, MYB, Proto oncogene c Myb, Transcriptional activator Myb

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Galerie de données de validation

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  WB analysis using 17800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17800-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17800-1-AP (c-Myb antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 17800-1-AP

  IP Result of anti-c-Myb (IP:17800-1-AP, 4ug; Detection:17800-1-AP 1:300) with HL-60 cells lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Jurkat, cellules MOLT-4
• Résultats positifs en IP: cellules HL-60

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

17800-1-AP cible MYB/c-Myb dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYB/c-Myb Protéine recombinante Ag12021
• Nom complet: v-myb myeloblastosis viral oncogene homolog (avian)
• Masse moléculaire calculée: 640 aa, 72 kDa
• Poids moléculaire observé: 40-50 kDa, 72-80 kDa
• Numéro d’acquisition GenBank: BC064955
• Symbole du gène: MYB
• Identification du gène (NCBI): 4602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.

Publications

Species	Application	Title
human	WB	Nat Commun; NLRP3 phosphorylation in its LRR domain critically regulates inflammasome assembly.; Authors - Tingting Niu
mouse	WB	Nat Commun; Tcf1 and Lef1 provide constant supervision to mature CD8+ T cell identity and function by organizing genomic architecture.; Authors - Qiang Shan
human	WB,CoIP	RNA Biol; SND1 acts as an anti-apoptotic factor via regulating the expression of lncRNA UCA1 in hepatocellular carcinoma.; Authors - Xiaoteng Cui
human	IF,WB	Front Endocrinol (Lausanne); KRT17 from skin cells with high glucose stimulation promotes keratinocytes proliferation and migration; Authors - Peng Zhou
human	WB	Front Med (Lausanne); Lidocaine and Bupivacaine Downregulate MYB and DANCR lncRNA by Upregulating miR-187-5p in MCF-7 Cells.; Authors - Chiao-Yi Lin
mouse	WB	Cancer Genomics Proteomics; Myb Repression Mediates Stat5b-knockdown-induced Apoptosis and Inhibits Proliferation of Glioblastoma Stem Cells; Authors - Chiami Moyama