Anticorps Recombinant de lapin anti-MYB/c-Myb

MYB/c-Myb Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230179C11

N° de cat : 82804-2-RR

Synonymes

MYB, c-Myb, Transcriptional activator Myb, Proto-oncogene c-Myb, Proto oncogene c Myb

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Galerie de données de validation

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  WB analysis using 82804-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82804-2-RR (MYB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 82804-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MYB/c-Myb Recombinant antibody (82804-2-RR, Clone:230179C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HT-29, cellules Jurkat, cellules MOLT-4
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82804-2-RR cible MYB/c-Myb dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MYB/c-Myb Protéine recombinante Ag12021
• Nom complet: v-myb myeloblastosis viral oncogene homolog (avian)
• Masse moléculaire calculée: 640 aa, 72 kDa
• Poids moléculaire observé: 40-50 kDa, 72-80 kDa
• Numéro d’acquisition GenBank: BC064955
• Symbole du gène: c-Myb
• Identification du gène (NCBI): 4602
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MYB protein is a transcription factor that plays a key role in regulating the proliferation/differentiation of progenitor cells in bone marrow, colonic crypts, and neurogenic niches [PMID:18574464]. It is involved in the regulation of epithelial-to-mesenchymal transition (EMT) and invasion in neuroblastoma, colon carcinoma, and embryonic kidney cells through the upregulation of the transcription repressor Slug [PMID:20622260]. It also a DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3', and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. MYB exists some isoforms with MV 85, 75, 72, 68, 63 and 40 kDa.

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