Anticorps Polyclonal de lapin anti-MYBBP1A

• Phare
• Validé par KD/KO

MYBBP1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14524-1-PBS

Synonymes

Myb binding protein 1A, Myb-binding protein 1A, PAP2

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Galerie de données de validation

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  WB analysis using 14524-1-AP (same clone as 14524-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 14524-1-AP (same clone as 14524-1-PBS)

  WB result of MYBBP1A antibody (14524-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYBBP1A transfected HEK-293 cells. This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 14524-1-AP (same clone as 14524-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14524-1-AP (MYBBP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 14524-1-AP (same clone as 14524-1-PBS)

  IP result of anti-MYBBP1A (IP:14524-1-AP, 4ug; Detection:14524-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14524-1-AP (same clone as 14524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14524-1-AP (same clone as 14524-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 14524-1-AP (MYBBP1A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14524-1-AP (same clone as 14524-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using MYBBP1A antibody 14524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14524-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14524-1-PBS cible MYBBP1A dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYBBP1A Protéine recombinante Ag6008
• Nom complet: MYB binding protein (P160) 1a
• Masse moléculaire calculée: 149 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC050546
• Symbole du gène: MYBBP1A
• Identification du gène (NCBI): 10514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].

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