Anticorps Monoclonal anti-MYBBP1A

• Phare
• Validé par KD/KO

MYBBP1A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H2D10

N° de cat : 67996-1-PBS

Synonymes

1H2D10, Myb binding protein 1A, Myb-binding protein 1A, PAP2

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Galerie de données de validation

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  WB analysis using 67996-1-Ig (same clone as 67996-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67996-1-Ig (same clone as 67996-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67996-1-Ig (same clone as 67996-1-PBS)

  WB result of MYBBP1A antibody (67996-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYBBP1A transfected HEK-293 cells. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67996-1-Ig (same clone as 67996-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67996-1-Ig (MYBBP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67996-1-Ig (MYBBP1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 67996-1-Ig (same clone as 67996-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MYBBP1A antibody (67996-1-Ig, Clone: 1H2D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67996-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67996-1-PBS cible MYBBP1A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYBBP1A Protéine recombinante Ag6008
• Nom complet: MYB binding protein (P160) 1a
• Masse moléculaire calculée: 149 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC050546
• Symbole du gène: MYBBP1A
• Identification du gène (NCBI): 10514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The protooncogene MYB is predominantly expressed in immature hemopoietic cells where it has an essential role in hemopoietic cell proliferation and differentiation. Oncogenically activated forms of MYB is generally N- and/or C-terminal truncations of the normal MYB protein. Removal of the C terminus of MYB disrupts or deletes a region termed the negative regulatory domain (NRD), resulting in an increase in DNA binding, transactivation, and transformation by MYB. One feature of the NRD is a leucine zipper-like motif [PMID: 8302594]. Murine Myb-binding protein-1a (MYBBP1A), originally called P160, was identified by its ability to interact specifically with Myb via this leucine zipper-like motif. MYBBP1A modulates MYB activity upon binding to the MYB NRD [PMID: 10644447, 9447996].

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