Anticorps Polyclonal de lapin anti-B-Myb

• Phare
• Validé par KD/KO

B-Myb Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (1)

N° de cat : 18896-1-AP

Synonymes

B MYB, BMYB, B-Myb, Myb like protein 2, Myb related protein B, MYBL2

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Galerie de données de validation

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  WB analysis of HeLa using 18896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18896-1-AP (B-Myb antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18896-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18896-1-AP (B-Myb antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18896-1-AP

  IP result of anti-B-Myb (IP:18896-1-AP, 4ug; Detection:18896-1-AP 1:300) with HeLa cells lysate 1520ug.

• 

  IHC staining of human prostate cancer using 18896-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18896-1-AP (B-Myb antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 18896-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18896-1-AP (B-Myb antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• ChIP: See 3 publications below

Informations sur le produit

18896-1-AP cible B-Myb dans les applications de WB, IHC, IF, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B-Myb Protéine recombinante Ag13525
• Nom complet: v-myb myeloblastosis viral oncogene homolog (avian)-like 2
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC007585
• Symbole du gène: B Myb
• Identification du gène (NCBI): 4605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYBL2, also named as BMYB, is a 700 amino acid protein, which is phosphorylated by cyclin A/CDK2 during S-phase. MYBL2 is a transcription factor involved in the regulation of cell survival, proliferation, and differentiation. The calculated molecular weight of MYBL2 is 79 kDa, but modified MYBL2 is about 90 kDa (PMID: 11418655).

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
human	WB	Oxid Med Cell Longev; LncRNA-MALAT1 Regulates Cancer Glucose Metabolism in Prostate Cancer via MYBL2/mTOR Axis.; Authors - Xiangyu Mu
human,mouse	WB	J Virol; Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection.; Authors - Matthew S Fuller
human	WB,IHC,IP	Cancer Gene Ther; A targetable MYBL2-ATAD2 axis governs cell proliferation in ovarian cancer; Authors - Qun Liu ; KO Validated
mouse	WB	Mol Cells; Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway.; Authors - Jianxiong Li
human	WB	Mediators Inflamm; Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2; Authors - Junqi Fu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

ARUNASALAM (Verified Customer) (07-23-2025)

This antibody works very well in western blotting even with higher dilutions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Cancer Cell Lines

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