Anticorps Polyclonal de lapin anti-MYBPC3

MYBPC3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19977-1-PBS

Synonymes

Myosin-binding protein C, cardiac-type, MYBPC 3, C-protein, cardiac muscle isoform, Cardiac MyBP-C, Cardiac MyBP C

---

Galerie de données de validation

• 

  WB analysis using 19977-1-AP (same clone as 19977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 19977-1-AP (same clone as 19977-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 19977-1-AP (same clone as 19977-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19977-1-AP (MYBPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19977-1-AP (MYBPC3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 19977-1-AP (same clone as 19977-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 19977-1-AP (MYBPC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 19977-1-AP (same clone as 19977-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using MYBPC3 antibody (19977-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19977-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

19977-1-PBS cible MYBPC3 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin binding protein C, cardiac
• Masse moléculaire calculée: 141 kDa
• Poids moléculaire observé: 141-150 kDa
• Numéro d’acquisition GenBank: NM_000256
• Symbole du gène: MYBPC3
• Identification du gène (NCBI): 4607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYBPC3 belongs to the immunoglobulin superfamily and MyBP family. MYBPC3 located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4). The antibody detects the full length form and the degradation bands around 28-54 kDa in SDS-PAGE.(PMID: 22076249)

{{ptg:RelatedPrimaryAntibodies}}