- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-c-MYC
c-MYC Polyclonal Antibody for FC, IF, IP, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (4)
Applications
WB, IP, IF, FC, EMSA, CoIP, ChIP, ELISA
Conjugaison
Non conjugué
569
N° de cat : 10828-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HT-29, cellules A549, cellules BxPC-3, cellules HeLa, cellules HepG2, cellules HL-60, cellules Jurkat, cellules Jurkat et cellules Raji, cellules Raji, cellules SH-SY5Y, cellules SW-1990, tissu placentaire humain |
Résultats positifs en IP | cellules MCF-7, |
Résultats positifs en IF | Saos-2 cells, cellules HEK-293, cellules U2OS |
Résultats positifs en cytométrie | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 21 publications below |
WB | See 516 publications below |
IF | See 33 publications below |
IP | See 19 publications below |
CoIP | See 14 publications below |
ChIP | See 10 publications below |
Informations sur le produit
10828-1-AP cible c-MYC dans les applications de WB, IP, IF, FC, EMSA, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | rat, Humain, porc, poulet, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | c-MYC Protéine recombinante Ag1263 |
Nom complet | v-myc myelocytomatosis viral oncogene homolog (avian) |
Masse moléculaire calculée | 49 kDa |
Poids moléculaire observé | 62-65 kDa, 50 kDa |
Numéro d’acquisition GenBank | BC000141 |
Symbole du gène | MYC |
Identification du gène (NCBI) | 4609 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Function
c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.
Tissue specificity
c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.
Involvement in disease
· Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.
· Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.
Isoforms
There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.
Post-translational modifications
c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.
Cellular localization
c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.
Protocole
Product Specific Protocols | |
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WB protocol for c-MYC antibody 10828-1-AP | Download protocol |
IF protocol for c-MYC antibody 10828-1-AP | Download protocol |
IP protocol for c-MYC antibody 10828-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration
| ||
Signal Transduct Target Ther Overexpression of CIP2A is associated with poor prognosis in multiple myeloma. | ||
Gastroenterology Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis | ||
Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.
| ||
Cell Metab Efferocytosis induces macrophage proliferation to help resolve tissue injury.
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Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Molly (Verified Customer) (05-02-2023) | The antibody worked well for western blotting following the recommended protocol.
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FH Anna-Maria (Verified Customer) (08-13-2019) | This antibody worked as expected for western blotting
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FH Christine (Verified Customer) (11-13-2018) | The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.
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FH Claudio (Verified Customer) (12-14-2017) | We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.
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