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Anticorps Polyclonal de lapin anti-MYC

MYC Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 26197-1-AP

Synonymes

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64

Galerie de données de validation

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  • Western Blot (WB) analysis of HeLa cells using MYC Polyclonal antibody (26197-1-AP)

    WB analysis of HeLa using 26197-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26197-1-AP (MYC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBtissu embryonnaire de souris, cellules HeLa, tissu placentaire humain

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

26197-1-AP cible MYC dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène MYC Protéine recombinante Ag24299
Nom complet v-myc myelocytomatosis viral oncogene homolog (avian)
Masse moléculaire calculée 49 kDa
Poids moléculaire observé 65-70 kDa
Numéro d’acquisition GenBankBC000141
Symbole du gène MYC
Identification du gène (NCBI) 4609
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. This antibody is a rabbit polyclonal antibody raised against recombinant protein of human MYC.The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC [PMID: 12189186]

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