Anticorps Recombinant de lapin anti-MYC tag

MYC tag Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

241611D1

N° de cat : 80469-2-RR

Synonymes

241611D1

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Galerie de données de validation

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  WB analysis of Transfected HEK-293T using 80469-2-RR

  Transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 80469-2-RR (MYC tag antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IP experiment of Transfected HEK-293 using 80469-2-RR

  IP result of anti-MYC tag (IP:80469-2-RR, 4ug; Detection:80469-2-RR 1:5000) with Transfected HEK-293 cells lysate 1600 ug.

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  IF Staining of Transfected HEK-293 using 80469-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using MYC tag antibody (80469-2-RR, Clone: 241611D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Transfected HEK-293 using 80469-2-RR

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug MYC tag Recombinant antibody (80469-2-RR, Clone:241611D1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 80469-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80469-2-RR against MYC tag were performed. The affinity constant is 0.134 nM.

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Applications testées

• Résultats positifs en WB: Transfected HEK-293T cells,
• Résultats positifs en IP: cellules HEK-293 transfectées,
• Résultats positifs en IF/ICC: cellules HEK-293 transfectées,
• Résultats positifs en FC (Intra): cellules HEK-293 transfectées,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80469-2-RR cible MYC tag dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MYC tag Protéine recombinante Ag9409
• Nom complet: Myc tag
• Symbole du gène: Myc tag
• Identification du gène (NCBI): 99
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The myc-tag is a short synthetic polypeptide sequence derived from c-MYC protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL. MYC tag is a part of c-MYC protein. So there's weaker 62-65 kDa bands for endogenous c-MYC protein. What is the molecular weight of myc? 1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code). What are the applications for myc tag? The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106). What is the structure of myc tag? The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.

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