Anticorps Polyclonal de lapin anti-MYCN

• Phare
• Validé par KD/KO

MYCN Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10159-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human placenta using 10159-2-AP (same clone as 10159-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 10159-2-AP (same clone as 10159-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of SKOV-3 using 10159-2-AP (same clone as 10159-2-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 10159-2-AP (same clone as 10159-2-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 10159-2-AP (same clone as 10159-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10159-2-AP (same clone as 10159-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 10159-2-AP (same clone as 10159-2-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10159-2-AP (MYCN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 10159-2-AP (same clone as 10159-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP (MYCN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 10159-2-AP (same clone as 10159-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10159-2-AP (MYCN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10159-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10159-2-PBS cible MYCN dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYCN Protéine recombinante Ag0193
• Nom complet: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC002712
• Symbole du gène: MYCN
• Identification du gène (NCBI): 4613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )

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