Anticorps Recombinant de lapin anti-MYCN

MYCN Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242406D8

N° de cat : 84886-3-RR

Synonymes

242406D8, bHLHe37, MODED, N myc, N myc proto oncogene protein

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Galerie de données de validation

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  WB analysis using 84886-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84886-3-RR (MYCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 84886-3-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84886-3-RR (MYCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 84886-3-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MYCN Recombinant antibody (84886-3-RR, Clone:242406D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: IMR-32 cells, tissu placentaire humain
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84886-3-RR cible MYCN dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MYCN Protéine recombinante Ag27224
• Nom complet: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: ~65 kDa
• Numéro d’acquisition GenBank: BC002712
• Symbole du gène: MYCN
• Identification du gène (NCBI): 4613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses. The calculated molecuar weight of MYCN is 50 kDa, but Phosphorylated MYCN is about 65-70 kDa. MYCN exsits two isoforms and MV of isoform is respectively 65 kDa and 45 kDa. (PMID: 19615087 )

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