Anticorps Polyclonal de lapin anti-MYD88

• Phare
• Validé par KD/KO

MYD88 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

158

Avis (2)

N° de cat : 23230-1-AP

Synonymes

MYD88, MYD88D

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Galerie de données de validation

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  WB analysis using 23230-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23230-1-AP (MYD88 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23230-1-AP

  WB result of MYD88 antibody (23230-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYD88 transfected HepG2 cells.

• 

  WB analysis using 23230-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23230-1-AP (MYD88 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23230-1-AP

  WB result of MYD88 antibody (23230-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYD88 transfected MCF-7 cells.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules A549, cellules HepG2, cellules K-562, cellules MCF-7, cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 146 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

23230-1-AP cible MYD88 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, canin, Humain, porc, poulet, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYD88 Protéine recombinante Ag19770
• Nom complet: myeloid differentiation primary response gene (88)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC013589
• Symbole du gène: MYD88
• Identification du gène (NCBI): 4615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myeloid differentiation primary response 88 (MYD88) is an adapter protein critical to the innate and adaptive immune response.

 What is the molecular weight of MYD88?

The molecular weight of MYD88 is 33 kDa.

 What is the cellular localization of MYD88?

The subcellular localization of MYD88 is largely confined to the cytoplasm as condensed forms or aggregated structures.

 What is the role of MYD88 in the IL-1R signaling pathway?

MYD88 plays a major role in the inflammatory signaling pathways downstream of Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) families. MYD88 links these receptors to IL-1R-associated kinases (IRAK), such as IRAK1 and IRAK2, via protein-protein interactions. The C-terminal TIR domain of MYD88 mediates the interaction with the receptors, whereas the N-terminal death domain of MYD88 associates with IRAK family members (PMID: 25580251). MYD88 acts via its intermediate domain to phosphorylate and thus activate IRAK1, IRAK2, IRF7, and TRAF6 to trigger NF-kappa-B signaling and cytokine secretion as part of the inflammatory response (PMID: 19679662).

 What is MYD88's involvement in disease?

Defects in MYD88 due to deficiency of the protein leads to recurrent pyogenic bacterial infections, including invasive pneumococcal disease. Patients usually die between 1 and 11 months of age, but surviving patients are otherwise healthy with normal resistance to other microbes (PMID: 18669862). Mutations in the MYD88 gene also lead to the development of cancers such as lymphoma (PMID: 21179087) and some autoimmune disorders like ulcerative colitis (PMID: 24189845).

Publications

Species	Application	Title
	WB	Adv Mater; Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages; Authors - Shuodan Huang
	WB	Mol Cell; CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity; Authors - Guiheng Zhang ; KO Validated
	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
	WB	J Crohns Colitis; A Molecular Targeted Immunotherapeutic Strategy for Ulcerative Colitis via Dual-targeting Nanoparticles Delivering miR-146b to Intestinal Macrophages.; Authors - Feihong Deng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rouba (Verified Customer) (07-05-2022)

Antibody was diluted to the 1/100 in blocking solution and incubated ON at 4 degrees celcius

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Myoblasts

MYD88 Antibody Immunofluorescence validation (1/100 dilution) in Myoblasts (Cat no:23230-1-AP)

FH

Cassand (Verified Customer) (10-22-2021)

Nice antibody works good.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Gut

MYD88 Antibody Immunohistochemistry validation (1:300 dilution) in Gut (Cat no:23230-1-AP)