Anticorps Monoclonal anti-MYD88

MYD88 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

22

CloneNo.

2E10D3

N° de cat : 67969-1-Ig

Synonymes

MYD88, MYD88D

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Galerie de données de validation

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  WB analysis using 67969-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HL-60 using 67969-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67969-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67969-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 67969-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit spleen using 67969-1-Ig

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67969-1-Ig (MYD88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67969-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67969-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67969-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67969-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67969-1-Ig (MYD88 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 67969-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using MYD88 antibody (67969-1-Ig, Clone: 2E10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HL-60, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3, cellules Raji, tissu splénique de lapin, tissu splénique de porc
• Résultats positifs en IHC: tissu hépatique humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67969-1-Ig cible MYD88 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYD88 Protéine recombinante Ag27659
• Nom complet: myeloid differentiation primary response gene (88)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC013589
• Symbole du gène: MYD88
• Identification du gène (NCBI): 4615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the interleukin-1 receptor (IL-1R) and most Toll-like receptors (TLRs), which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis.

Publications

Species	Application	Title
rat	WB	J Control Release; Controlled extracellular vesicles release from aminoguanidine nanoparticle-loaded polylysine hydrogel for synergistic treatment of spinal cord injury; Authors - Shaoke Wang
rat	WB	Inflammation; Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro; Authors - Kai Li
mouse	WB	Front Bioeng Biotechnol; Polyvinylpyrrolidone-Modified Taxifolin Liposomes Promote Liver Repair by Modulating Autophagy to Inhibit Activation of the TLR4/NF-κB Signaling Pathway.; Authors - Qiteng Ding
human	WB	Biomater Adv; Monosodium urate crystals with controlled shape and aspect ratio for elucidating the pathological progress of acute gout.; Authors - Chen Chen
mouse	WB	Nutrients; Hawk Tea Flavonoids as Natural Hepatoprotective Agents Alleviate Acute Liver Damage by Reshaping the Intestinal Microbiota and Modulating the Nrf2 and NF-κB Signaling Pathways; Authors - Ting Xu
mouse	WB	Int J Mol Sci; Methyl Gallate Alleviates Acute Ulcerative Colitis by Modulating Gut Microbiota and Inhibiting TLR4/NF-κB Pathway; Authors - Ping Zhou