Anticorps Polyclonal de lapin anti-MYEF2

MYEF2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 16051-1-AP

Synonymes

HsT18564, KIAA1341, MEF 2, MST156, MSTP156, MyEF 2, MYEF2, myelin expression factor 2

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Galerie de données de validation

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  WB analysis using 16051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16051-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16051-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16051-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16051-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16051-1-AP (MYEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16051-1-AP

  IP Result of anti-MYEF2 (IP:16051-1-AP, 4ug; Detection:16051-1-AP 1:2000) with Jurkat cells lysate 2800ug.

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  IHC staining of human breast cancer using 16051-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16051-1-AP (MYEF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16051-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16051-1-AP (MYEF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16051-1-AP

  Immunofluorescent analysis of HepG2 cells, using MYEF2 antibody 16051-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 16051-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug MYEF2 antibody (16051-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

16051-1-AP cible MYEF2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYEF2 Protéine recombinante Ag9010
• Nom complet: myelin expression factor 2
• Masse moléculaire calculée: 600 aa, 64 kDa
• Poids moléculaire observé: 61-64 kDa
• Numéro d’acquisition GenBank: BC014533
• Symbole du gène: MYEF2
• Identification du gène (NCBI): 50804
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB, IHC, CoIP	Cell Rep; PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination.; Authors - Yan Wang
human	WB,IHC	BMC Gastroenterol; Expression and function of myelin expression factor 2 in hepatocellular carcinoma; Authors - Peng Zhang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wael (Verified Customer) (05-16-2023)

Hek293 cells were fractionated into nuclear and cytoplasmic fractions. specific band for MYEF2 between 55 and 100kd, enriched in the Nuclear fraction. Lanes order: 1-ladder 2-Nuclear fraction 3-Cytoplasmic frcation

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

MYEF2 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:16051-1-AP)