Anticorps Monoclonal anti-MYH1

MYH1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G10H9

N° de cat : 67299-1-PBS

Synonymes

1G10H9, MyHC-2x, MyHC-IIx/d, Myosin 1, Myosin-1

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  1 μg human skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  1 μg pig skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  1 μg mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 67299-1-Ig (MYH1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67299-1-Ig (MYH1 antibody) at dilution of 1:80000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 67299-1-Ig (same clone as 67299-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using MYH1 antibody (67299-1-Ig, Clone: 1G10H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67299-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67299-1-PBS cible MYH1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYH1 Protéine recombinante Ag17129
• Nom complet: myosin, heavy chain 1, skeletal muscle, adult
• Masse moléculaire calculée: 1939 aa, 223 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC114545
• Symbole du gène: MYH1
• Identification du gène (NCBI): 4619
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

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