Anticorps Polyclonal de lapin anti-MYH1-motor domain

MYH1-motor domain Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22282-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 22282-1-AP (same clone as 22282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

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  FC experiment of C2C12 using 22282-1-AP (same clone as 22282-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22282-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22282-1-PBS cible MYH1-motor domain dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 1, skeletal muscle, adult
• Masse moléculaire calculée: 223 kDa
• Poids moléculaire observé: 223 kDa
• Numéro d’acquisition GenBank: NM_005963
• Symbole du gène: MYH1
• Identification du gène (NCBI): 4619
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

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