Anticorps Recombinant de lapin anti-MYH10

MYH10 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250791D5

N° de cat : 86157-3-PBS

Synonymes

Myosin 10, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Myosin-10, NMMHC II-b

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Galerie de données de validation

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  WB analysis using 86157-3-RR (same clone as 86157-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

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  WB analysis using 86157-3-RR (same clone as 86157-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86157-3-RR (MYH10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86157-3-RR (MYH10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 86157-3-RR (MYH10 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 86157-3-RR (same clone as 86157-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using MYH10 antibody (86157-3-RR, Clone: 250791D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86157-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86157-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86157-3-RR against Human MYH10 were performed. The affinity constant is 4.58 nM.

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Informations sur le produit

86157-3-PBS cible MYH10 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MYH10 Protéine recombinante Ag16113
• Nom complet: myosin, heavy chain 10, non-muscle
• Masse moléculaire calculée: 1985 aa, 230 kDa
• Poids moléculaire observé: 229 kDa
• Numéro d’acquisition GenBank: BC150634
• Symbole du gène: MYH10
• Identification du gène (NCBI): 4628
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

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