Anticorps Polyclonal de lapin anti-SMMHC

SMMHC Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 18569-1-AP

Synonymes

MYH11, Myosin-11, Myosin heavy chain 11, Myosin 11, KIAA0866

---

Galerie de données de validation

• 

  WB analysis of mouse colon using 18569-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 18569-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 18569-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat uterus using 18569-1-AP

  rat uterus tissue were subjected to SDS PAGE followed by western blot with 18569-1-AP (SMMHC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human normal colon using 18569-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18569-1-AP (SMMHC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu de côlon de souris, tissu utérin de rat, tissu utérin de souris, tissu vésical de souris
• Résultats positifs en IHC: human normal colon, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18569-1-AP cible SMMHC dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMMHC Protéine recombinante Ag13391
• Nom complet: myosin, heavy chain 11, smooth muscle
• Masse moléculaire calculée: 1938 aa, 224 kDa
• Poids moléculaire observé: 227 kDa
• Numéro d’acquisition GenBank: BC101677
• Symbole du gène: SMMHC/MYH11
• Identification du gène (NCBI): 4629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Am Heart Assoc; LINC01278 Sponges miR-500b-5p to Regulate the Expression of ACTG2 to Control Phenotypic Switching in Human Vascular Smooth Muscle Cells During Aortic Dissection.; Authors - Weitie Wang
rat	WB	Front Pharmacol; Theaflavin-3,3'-Digallate from Black Tea Inhibits Neointima Formation Through Suppression of the PDGFRβ Pathway in Vascular Smooth Muscle Cells.; Authors - Yichen Wu
human	WB	Front Med (Lausanne); Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.; Authors - Fang He
human	IHC	BMC Cancer; Identification of a mitophagy-related gene signature for predicting overall survival and response to immunotherapy in rectal cancer; Authors - Jian Yang
human	WB	Ultrasound Med Biol; 5-Aminolevulinic Acid-Mediated Sonodynamic Therapy Promotes Phenotypic Switching from Dedifferentiated to Differentiated Phenotype via Reactive Oxygen Species and p38 Mitogen-Activated Protein Kinase in Vascular Smooth Muscle Cells.; Authors - Juhua Dan
mouse	IHC	Cell Signal; Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production.; Authors - Daorong Pan

{{ptg:RelatedPrimaryAntibodies}}