Anticorps Polyclonal de lapin anti-MYH14

MYH14 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 20716-1-AP

Synonymes

MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C, Non muscle myosin heavy chain IIc, non-muscle II c

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Galerie de données de validation

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  WB analysis using 20716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 20716-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 20716-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 20716-1-AP

  IP Result of anti-MYH14 (IP:20716-1-AP, 4ug; Detection:20716-1-AP 1:2000) with mouse kidney tissue lysate 4800ug.

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  IF Staining of HeLa using 20716-1-AP

  Immunofluorescent analysis of Hela cells, using MYH14 antibody 20716-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HT-29 using 20716-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human MYH14 (20716-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, tissu de côlon de souris, tissu de muscle squelettique humain, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Informations sur le produit

20716-1-AP cible MYH14 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 14
• Masse moléculaire calculée: 228 kDa
• Poids moléculaire observé: 228 kDa
• Numéro d’acquisition GenBank: NM_024729
• Symbole du gène: MYH14
• Identification du gène (NCBI): 79784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions.; Authors - Liyin Yu
human	WB	J Cell Biol; Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane; Authors - Florian Rouaud
human	IHC	Clin Genet; Mutations of MYH14 Are Associated to Anorectal Malformations with Recto-perineal Fistulas in a Small Subset of Chinese Population.; Authors - Zhongxian Zhu
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
mouse	IF	Mol Biol Cell; Nonmuscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli.; Authors - Colbie R Chinowsky
mouse	WB,IF	Life (Basel); Mass Spectrometric Profiling of Extraocular Muscle and Proteomic Adaptations in the mdx-4cv Model of Duchenne Muscular Dystrophy.; Authors - Stephen Gargan