Anticorps Polyclonal de lapin anti-MYH14

MYH14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20716-1-PBS

Synonymes

Myosin 14, myosin, MHC16, KIAA2034, FP17425

---

Galerie de données de validation

• 

  WB analysis using 20716-1-AP (same clone as 20716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 20716-1-AP (same clone as 20716-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 20716-1-AP (same clone as 20716-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20716-1-AP (MYH14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 20716-1-AP (same clone as 20716-1-PBS)

  IP result of anti-MYH14 (IP:20716-1-AP, 4ug; Detection:20716-1-AP 1:2000) with mouse kidney tissue lysate 4800ug. This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 20716-1-AP (same clone as 20716-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20716-1-AP (MYH14 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20716-1-AP (same clone as 20716-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MYH14 antibody (20716-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20716-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

20716-1-PBS cible MYH14 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 14
• Masse moléculaire calculée: 228 kDa
• Poids moléculaire observé: 228 kDa
• Numéro d’acquisition GenBank: NM_024729
• Symbole du gène: MYH14
• Identification du gène (NCBI): 79784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.

{{ptg:RelatedPrimaryAntibodies}}