Anticorps Monoclonal anti-MYH14

• Phare
• Validé par KD/KO

MYH14 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B4C9

N° de cat : 66825-1-PBS

Synonymes

myosin, MHC16, KIAA2034, FP17425, DFNA4

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Galerie de données de validation

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  WB analysis of COLO 320 using 66825-1-Ig (same clone as 66825-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66825-1-Ig (same clone as 66825-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 66825-1-Ig (same clone as 66825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66825-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66825-1-PBS cible MYH14 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYH14 Protéine recombinante Ag28063
• Nom complet: myosin, heavy chain 14
• Masse moléculaire calculée: 228 kDa
• Poids moléculaire observé: 200-230 kDa
• Numéro d’acquisition GenBank: NM_001077186
• Symbole du gène: MYH14
• Identification du gène (NCBI): 79784
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).

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