Anticorps Polyclonal de lapin anti-MYH6

• Phare
• Validé par KD/KO

MYH6 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22281-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 22281-1-AP (same clone as 22281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22281-1-AP (MYH6 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  IP result of anti-MYH6 (IP:22281-1-AP, 5ug; Detection:22281-1-AP 1:300) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 22281-1-AP (same clone as 22281-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 22281-1-AP (MYH6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22281-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22281-1-PBS cible MYH6 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 6, cardiac muscle, alpha
• Masse moléculaire calculée: 224 kDa
• Poids moléculaire observé: 200-220 kDa
• Numéro d’acquisition GenBank: NM_002471
• Symbole du gène: MYH6
• Identification du gène (NCBI): 4624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)

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