Anticorps Monoclonal anti-MYH9

• Phare
• Validé par KD/KO

MYH9 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(11)

CloneNo.

5D9D2

N° de cat : 60233-1-Ig

Synonymes

5D9D2, Myosin 9, Myosin heavy chain 9, Myosin heavy chain, non-muscle IIa, Myosin-9

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Galerie de données de validation

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  WB analysis of mouse kidney using 60233-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60233-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 60233-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 60233-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 60233-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig pancreas using 60233-1-Ig

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 60233-1-Ig

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 60233-1-Ig (MYH9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 60233-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of U2OS using 60233-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules A431, cellules U2OS, tissu de côlon de souris, tissu pancréatique de porc, tissu placentaire humain, tissu rénal de porc, tissu rénal humain, tissu splénique de porc
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

60233-1-Ig cible MYH9 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYH9 Protéine recombinante Ag1612
• Nom complet: myosin, heavy chain 9, non-muscle
• Masse moléculaire calculée: 227 kDa
• Poids moléculaire observé: 230 kDa, 160 kDa
• Numéro d’acquisition GenBank: BC011915
• Symbole du gène: MYH9
• Identification du gène (NCBI): 4627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

Publications

Species	Application	Title
human	WB	Cell Stem Cell; Mechano-oncogenic cytoskeletal remodeling drives leukemic transformation with mitochondrial vesicle-mediated STING activation; Authors - Zemin Song
human	WB	Nat Commun; Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.; Authors - Pasquale Cervero
human	WB,IHC,IF	EBioMedicine; PMID: 31594754; Authors - Yiyi Liu ; KD Validated
human	WB,IF,CoIP	Cell Death Differ; Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-β-catenin-STAT3 signaling; Authors - Shunfeng Hu ; KD Validated
mouse,human	WB,IHC,IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
mouse	IF	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang

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