Anticorps Monoclonal anti-MYH9

• Phare
• Validé par KD/KO

MYH9 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5D9D2

N° de cat : 60233-1-PBS

Synonymes

5D9D2, Myosin 9, Myosin heavy chain 9, Myosin heavy chain, non-muscle IIa, Myosin-9

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Galerie de données de validation

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  WB analysis of mouse kidney using 60233-1-Ig (same clone as 60233-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  WB analysis using 60233-1-Ig (same clone as 60233-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 60233-1-Ig (same clone as 60233-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 60233-1-Ig (same clone as 60233-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  WB analysis of pig spleen using 60233-1-Ig (same clone as 60233-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  WB analysis of pig pancreas using 60233-1-Ig (same clone as 60233-1-PBS)

  pig pancreas tissue were subjected to SDS PAGE followed by western blot with 60233-1-Ig (MYH9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 60233-1-Ig (same clone as 60233-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 60233-1-Ig (MYH9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60233-1-Ig (same clone as 60233-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 60233-1-Ig (same clone as 60233-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 60233-1-Ig (MYH9 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60233-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60233-1-PBS cible MYH9 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYH9 Protéine recombinante Ag1612
• Nom complet: myosin, heavy chain 9, non-muscle
• Masse moléculaire calculée: 227 kDa
• Poids moléculaire observé: 230 kDa, 160 kDa
• Numéro d’acquisition GenBank: BC011915
• Symbole du gène: MYH9
• Identification du gène (NCBI): 4627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYH9 (Myosin-9) appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. MYH9 is a hexameric protein that consists of two heavy chains (~200 kDa) and two pairs of non-identical light chains (15-22 kDa), a pair of essential or alkali light chains, and a pair of regulatory light chains (PMID: 12800156).

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