Anticorps Polyclonal de lapin anti-MYL12B

MYL12B Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10324-1-AP

Synonymes

MLC 2, MLC 2A, MLC B, MLC20, MRLC2, MYL12B, MYLC2B, SHUJUN 1

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Galerie de données de validation

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  WB analysis using 10324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10324-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10324-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10324-1-AP (MYL12B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 10324-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10324-1-AP (MYL12B antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

10324-1-AP cible MYL12B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYL12B Protéine recombinante Ag0398
• Nom complet: myosin, light chain 12B, regulatory
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC004994
• Symbole du gène: MYL12B
• Identification du gène (NCBI): 103910
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYL12B is a regulatory subunit of myosin and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. There are two groups of residues on the MYL12B that are phosphorylated by distinct kinases and have contrasting effects on myosin II biophysical properties.Phosphorylation at Thr18/Ser19 essentially "activates" the myosin molecule to produce force. The second group of phosphorylated residues is at the N-terminus of the MYL12B at Ser1, Ser2 and Thr9 and causes inhibitory effect on myosin activity.(PMID: 22136066)

Publications

Species	Application	Title
human	WB	Mol Biol Cell; Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells.; Authors - Breckenridge Mark T MT
human	WB	Biochemistry (Mosc); Western Blotting-Based Quantitative Measurement of Myosin II Regulatory Light Chain Phosphorylation in Small Amounts of Non-muscle Cells.; Authors - O A Kazakova
human	WB,IF	BMC Cell Biol; Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells.; Authors - Beach Jordan R JR
human	WB	J Pept Sci; Design of peptidase-resistant peptide inhibitors of myosin light chain kinase.; Authors - Asker Y Khapchaev
human	WB	Biochemistry (Mosc); Long-Term Experimental Hyperglycemia Does Not Impair Macrovascular Endothelial Barrier Integrity and Function in vitro; Authors - Asker Y Khapchaev