Anticorps Monoclonal anti-MYL3

MYL3 Monoclonal Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

2E4B2

N° de cat : 66286-1-PBS

Synonymes

Myosin light chain 3, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MYL 3, MLC-lV/sb, MLC1V

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Galerie de données de validation

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  WB analysis using 66286-1-Ig (same clone as 66286-1-PBS)

  Various tissue lysates were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 66286-1-Ig (same clone as 66286-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 66286-1-Ig (MYL3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50642-3

  Sandwich ELISA standard curve of MP50642-3, Human MYL3 Monoclonal Matched Antibody Pair - PBS only. 66286-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24535. 66286-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP50642-3

  The mean MYL3 concentration was determined to be 23.06 ng/mL in mouse heart tissue extract based on a 6.9 mg/mL extract load and 3.2 ng/mL in mouse skeletal muscle tissue extract based on a 1.7 mg/mL extract load.

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  IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66286-1-Ig (same clone as 66286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 66286-1-Ig (same clone as 66286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66286-1-Ig (MYL3 Antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 66286-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50642-3

  Cytometric bead array standard curve of MP50642-3, MYL3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66286-1-PBS. Detection antibody: 66286-5-PBS. Standard:Ag24535. Range: 0.098-6.25 ng/mL.

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Informations sur le produit

66286-1-PBS cible MYL3 dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MYL3 Protéine recombinante Ag24535
• Nom complet: myosin, light chain 3, alkali; ventricular, skeletal, slow
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC009790
• Symbole du gène: MYL3
• Identification du gène (NCBI): 4634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

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